Made up to the mark with all the mobile phase to obtain
Produced up to the mark using the mobile phase to receive a answer containing 30 /ml of DIC. This option was used for the Amebae web estimation of DIC. The option is further diluted with mobile phase to receive 2.5 /ml MEF and 5 /ml of PCM, respectively. Both the solutions had been sonicated for ten min. Solutions were injected as per the above chromatographic circumstances and peak places have been recorded. The quantifications have been carried out by keeping these values towards the straight line equation of calibration curve.Results AND DISCUSSIONThe objective from the strategy improvement was to resolve chromatographic peaks for active drug components with less asymmetric factor. The mobile phase acetonitrile:20 mM potassium dihydrogen phosphate (70:30 v/v) adjusted to pH 4 using orthophosphoric acid was found to become satisfactory which gave 3 symmetric and wellresolved peaks for DIC, MEF and PCM. The retention times of DIC, MEF and PCM had been three.eight, 9.3 and 2.5 min, respectively (fig. 1). The resolution involving DIC, MEF and PCM was discovered to become far more than two, which indicates great separation of each of the compounds. The asymmetric aspects for DIC, MEF and PCM were 1.36, 1.14, 1.44, respectively. The mobile phase flow rate was maintained at 1 ml/min. Overlaid UV spectra of both the drugs showed that DIC, MEF and PCM absorbed appreciably at 220 nm, so detection was carried out at 220 nm.Fig. 1: Chromatogram of CBP/p300 Formulation normal PCM, DIC and MEF. Chromatogram of standard solutions of paracetamol (PCM, two.five min) dicyclomine (DIC, 3.eight min) and mefenamic acid (MEF, 9.three min) obtained in mobile phase. November – December 2014 Indian Journal of Pharmaceutical Sciencesijpsonline.comLinearity was evaluated by analysis of functioning regular options of DIC, MEF and PCM of five distinct concentrations and the method was located to be linear in the array of 1000 /ml for DIC, 0.050 /ml for MEF and 0.10 /ml for PCM, respectively. The regression information obtained are represented in Table 1. Instrument precision was determined by performing injection repeatability test along with the relative common deviation values for DIC, MEF and PCM have been found. The intraday and interday precision studies had been carried out for 3 concentrations of DIC, MEF and PCM plus the outcomes are reported in Table two. The accuracy in the system was determined by calculating recoveries of DIC, MEF and PCM by approach of normal addition. Recoveries have been found to become 97.839.26, 98.989.53 and 99.7900.16 for DIC, MEF and PCM, respectively (Table 2). Recovery studies had been performed in triplicate. The LOQ for DIC, MEF and PCM had been discovered to be 10, 0.05 and 0.1 /ml respectively. The LOD for DIC, MEF and PCM were located to be three, 0.0125 and 0.033 /ml respectively (Table 2). Robustness study was performed by deliberately changing the experimental circumstances like flow rate from 1 ml/min to 0.eight ml/min and 1.2 ml/min. The composition of mobile phase was changed varying the proportion of acetonitrile by five . In each the circumstances the recovery of all the drugs have been determined as well as the RSD was discovered to become significantly less than 2 . Remedy stability of DIC, MEF and PCM were evaluated at area temperature for 24 h. All of the drugs have been found to be stable having a recovery of more than 98 . System suitability parameters for instance the number of theoretical plates, resolution, and peak assymetry had been determined and reported in Table 2. The proposed strategy was effectively applied to the determination of DIC, MEF and PCM in their combined dosage type. The recovery was located.