s represent genes that exhibit twofold (padj 0.01) changes in expression in each species (D) Venn diagram with the quantity of genes that exhibit overlapping two fold (padj 0.01) changes in expression in F1 progeny of animals exposed to P. vranovensis BIGb0446 in every single species. (E) Normalized counts of reads matching DP web orthologs of rhy-1 in the F1 offspring of parents fed either E. coli HB101 or P. vranovensis BIGb0446. Data from Supplementary file 2. n = 3 replicates. (F) Typical fold change of 7587 single-copy ortholog genes in F1 progeny of C. elegans and C. briggsae parents grown at 300 mM NaCl when in comparison with parents grown at 50 mM NaCl. Average fold alter from three replicates. Red dots represent genes that exhibit twofold (padj 0.01) modifications in expression in both species. (G) Average fold alter of 7587 single-copy ortholog genes in F1 progeny of C. elegans and C. kamaaina parents grown at 300 mM NaCl when in comparison with parents grown at 50 mM NaCl. Average fold alter from 3 replicates. Red dots represent genes that exhibit twofold (padj 0.01) adjustments in expression in both species in each species. (H) Typical fold change of 7587 single-copy ortholog genes in F1 progeny of C. elegans and C. tropicalis parents grown at 300 mM NaCl when when compared with parents grown at 50 mM NaCl. Average fold transform from 3 replicates. Red dots represent genes exhibit twofold (padj 0.01) modifications in expression in both species. (I) Venn diagram from the number of genes that exhibit overlapping twofold (padj 0.01) modifications in expression in F1 progeny of animals grown at 300 mM NaCl in every species. (J) Normalized counts of reads matching orthologs of gpdh-1 inside the F1 progeny of parents grown at either 300 mM NaCl or 50 mM NaCl. Data from Supplementary file three. n = three replicates. (K) Typical fold transform for 7587 ortholog genes in F1 progeny of C. elegans parents fed P. vranovensis or exposed to 300 mM NaCl when compared to naive parents. Average fold adjust from 3 replicates. Red dots genes that transform in expression in CXCR3 custom synthesis response to both stresses. Blue dots genes that change in expression in response to only osmotic anxiety. Green dots genes that transform in expression in response to only P. vranovensis. (L) Average fold adjust of 7512 single-copy ortholog genes in F3 progeny of C. elegans and C. kamaaina fed P. vranovensis BIGb0446 when when compared with these fed E. coli HB101. Typical fold alter from three replicates. Blue dots represent genes that exhibited twofold (padj 0.01) modifications in expression in C. elegans. Green dots represent genes that exhibited twofold (padj 0.01) modifications in expression in C. kamaaina. (M) Typical fold alter of 7512 single-copy ortholog genes in F1 progeny of C. elegans and C. kamaaina parents grown at 300 mM NaCl when compared to parents grown at 50 mM NaCl. Average fold transform from three replicates. Green dots represent genes that exhibited twofold (padj 0.01) changes in expression in C. kamaaina. p 0.05, p 0.01, p 0.0001, p 0.0001. The on the internet version of this article contains the following figure supplement(s) for figure 2: Figure supplement 1. Variations in developmental timing are insufficient to explain a majority of your observed variations in gene expression in the offspring of stressed parents.Pairing gene expression and phenotypic data across species drastically enriches for genes needed for intergenerational adaptationsTo further probe how parental exposure to environmental stresses impacts offspring gene expre