Nces Brigelius et al. (2012) Yu et al. (2007) Gomez et al. (1997) Shao et al. (2012) Baker et al. (1998) Chen et al. (2011) Yamazaki et al. (2011)CDRSL FSL41 (14/34)DDRFSL74 (25/34)Valencia et al. (2012) Ruiz et al. (2011) Kim et al. (2011) Dactinomycin biological activity Sanz-Pamplona et al. (2011) Eletto et al. (2010) Calderwood et al. (2007) Rausa et al. (2004) Wolfrum et al. (2004) Tang et al. (2011) Schmedt et al. (2012) Zong et al. (2011) Arenzana et al. (1995) Fraczek et al. (2010) Haakensen et al. (2011) Tomita et al. (2009) Qiao et al. (2009) Zidane et al. (2012)HSP90BFSL82 (28/34)FOXAFSL29 (10/34)SDC1 C4BPA SCGB3A1 RPSAFSL FSL FSL FSL82 (28/34) 35 (12/34) 56 (19/34) 65 (22/34)ERGICFSL76 (47/62)Zhang et al. (2012) Nishikawa et al. (2007) Otte et al. (2002) Liu et al. (2011) Aida et al. (2011) Lee et al. (2010) Ma et PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28607003 al. (2007) Moon et al. (2010) Selmi et al.(2012) Lee et al. (2012) Matsui et al. (2012) Nakanishi et al. (2006) Soupene et al. (2012) Mansilla et al. (2009)DDX58 CCNDBP1 TMSB4X CXCL17 LPCATFSL FSL FSL FSL FSL47 (29/62) 47 (29/62) 37 (23/62) 45 (28/62) 47 (29/62): Down-regulation of the genes in lung cancer tissues compared with their adjacent nonmalignant lung tissues; : Up-regulation of the genes in lung cancer tissues compared with their adjacent nonmalignant lung tissues; #: Number of cases applicable/total number of cases examined.Wu et al. BMC Cancer 2013, 13:44 http://www.biomedcentral.com/1471-2407/13/Page 6 ofExpression of ERGIC3 protein in cultured cells and in lung cancer tissues by western blotExpression of the ERGIC3 protein was analyzed using western blot. Expression of ERGIC3 was increased in 67 (10/15) of the tumor cases (Figure 1A). Similarly, expression of ERGIC3 protein was increased in all three lung cancer cell lines by comparison with the BEAS-2B (Figure 1B).Subcellular localization of ERGIC3 protein in cultured cellsIn cultured cells, the subcellular localization of ERGIC3 was examined by immunofluorescence double-staining using markers of the Golgi apparatus and endoplasmic reticulum (ER). ERGIC3 was mainly located at the Golgi apparatus and ER in the lung cancer cell lines. Interestingly, ERGIC3 was distributed at the side of nucleus in EPLC-32M1, 801D, and NCI-H446 cells, but uniformly present around nucleus in SPCA-1, GLC-82 and A549 cells (Figure 2). We also found that ERGIC3 was co-localized with the epithelia mucin MUC1 and -Galactoside 2,6 Sialyltransferase (ST), which were principally located at the ER and Golgi apparatus in the cultured cells (Figure 2).Expression and localization of the ERGIC3 protein in lung cancer tissues by immunohistochemical stainingERGIC3 was positive in 89 of (31/35) NSCLCs, and strongly stained in 63 (22/35). Interestingly, the positive rate of AC (100 , 22/22) was higher than that of SCC (69 , 9/13). In poorly differentiated NSCLCs, 36 (4/11) cases were not stained. We noticed that all of negative specimens were poorly differentiated tumor cells, and the staining was decreased to the largest extent and even disappeared in poorly differentiated NSCLCs, compared with well and moderately NSCLCs (P <0.05). No correlations were observed between expression of ERGIC3 and the gender as well as smoking histories of the patients and TNM stage (Table 2).Effects of ERGIC3 expression on the proliferation of the epithelial cellsExpression and localization of the ERGIC3 protein was further investigated by immunohistochemistry in 35 cases of NSCLC. ERGIC3 was diffusely distribut.