Name :
Vaccinia Virus B18R / B19R Protein (His Tag)

Biological Activity :

Background :
B18R is a type I interferon (IFN)-binding protein, which is encoded by the B18R open reading frame in the WR (Western Reserve) strain of vaccinia virus. It is also known as B19R in the Copenhagen strain of Vaccinia. B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta. It has been shown that secreted B18R binds to uninfected and infected cells. It presents at the cell surface and protects cells from the antiviral state induced by IFN-alpha and IFN-beta. Binding of soluble recombinant B18R protects cultured cells from IFN and allows vaccinia virus replication.

Biological Activity :
Testing in progress

Expression Host :
VACV

Source :
Baculovirus-Insect Cells

Tag :

Protein Accession No. :
P25213

NCBI Gene ID :

Synonyms :

Synonyms :

Amino Acid Sequence :

Molecular Weight :
The secreted recombinant B18R consists of 343 amino acids and predicts a molecular mass of 39.86 KDa. The apparent molecular mass of the protein is approximately 46 KDa in SDS-PAGE under reducing conditions due to glycosylation.

Purity :
> 90 % as determined by SDS-PAGE

State of Matter :

Product Concentration :

Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method

Protein Construction :
A DNA sequence encoding the Vaccinia Virus B18R (P25213) (Met1-Glu351) was expressed with a C-terminal polyhistidine tag.

Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Synonyms :

References & Citations :
I Vancov. et al., 1998, J Gen Virol July. 79 (7): 1647-9. Colamonici OR. et al., 1995, J Biol Chem. 270 (27): 15974-8. Goebel SJ. et al., 1990, Virology 179: 247-66. Goebel SJ. et al., 2010, J Cell Biol. 190 (4): 533-9.

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