ANXA2 Protein | Proton-pump inhibitor proton-pump.com

Name :
ANXA2 Protein

Description :
ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib.

Species :
Human

Uniprotkb :
E. coli

Tag :
N-His

Synonyms :
LIP2PAP-IV, LPC2DP36, Annexin II, p36, Annexin A2, ANX2L4, Chromobindin-8, Lipocortin II, CAL1H, ANX2, PAP-IV, LPC2D, ANXA2

Construction :
Recombinant Human ANXA2 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Ser2-Asp339.[Accession |P07355]

Protein Purity :
> 95% as determined by Tris-Bis PAGE

Molecular Weight :
The protein has a predicted MW of 39.6 kDa same as Tris-Bis PAGE result.

Endotoxin :
Less than 1EU per μg by the LAL method.

Formulatione :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage :
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Research Background :
ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib.

References and Literature :
1. Zhang Y, et al. ANXA2 could act as a moderator of EGFR-directed therapy resistance in triple negative breast cancer. Biosci Biotechnol Biochem. 2018 Oct;82(10):1733-174doi: 10.1080/09168451.2018.1484275. Epub 2018 Jun 18. PMID: 29912633.

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